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cd80 pe  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd80 pe
    Cd80 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd80+pe/CD80+Antibody%2C+anti-mouse/pm41873526-404-17-62
    Average 93 stars, based on 182 article reviews
    cd80 pe - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Adipose tissue from oesophageal adenocarcinoma patients is differentially affected by chemotherapy and chemoradiotherapy regimens altering immune cell phenotype and cancer cell metabolism
    Article Snippet: .. Antibodies used for Mɸ staining included CD11b-FITC, CD11c-Vio-Blue, CD80-PE, CD206-APC-Vio770, CD86-PerCp-Vio700, HLA-DR-Vio-Green, TIM-3-APC, CD68-PE-Vio770, and CD163-PEVio615 (Miltenyi, Germany). ..

    Article Title: Robust Bioconjugated Antigens Induce Immune Responses Preventing Malaria Infection and its Transmission.
    Article Snippet: The concentration of IFN-γ in the supernatants was easured using a Rat IFN gamma SimpleStep ELISAKit (Abcam, K), and the results were analyzed with a BioTek microplate eader. he stimulated and unstimulated BMDCs were collected and tained with 7-Aminoactinomycin D (7-AAD; Miltenyi Biotech, SA), following the manufacturer’s instructions, to identify he live cells. .. The cells were then stained with the following luorochrome-conjugated antibodies for 30 min at 4◦C: anti-rat D11c-APC-Vio770, CD80-PE, CD86-PE-Vio770, and MHC-IIerCP-Vio700 to assess the maturation of the dendritic cells. fter washing the cells twice with 1x PBS buffer, they were ixed with 1% paraformaldehyde (ThermoFisher Scientific, USA) nd analyzed using a flow cytometer within 24 h. All the ntibodies were purchased from Miltenyi Biotech, USA, and sed according to the manufacturer’s instructions. ..

    Article Title: Adipose tissue from oesophageal adenocarcinoma patients is differentially affected by chemotherapy and chemoradiotherapy regimens altering immune cell phenotype and cancer cell metabolism.
    Article Snippet: Oesophageal adenocarcinoma (OAC) is a poor prognosis cancer with limited responses to standard of care treatments including chemotherapy and chemoradiotherapy.. OAC has one of the strongest associations with obesity, its anatomical location surrounded by visceral adipose tissue has been postulated to intensify this association.. Adipose tissue is a regulatory organ with many unknown downstream functions, including its direct response to chemotherapy and radiotherapy.

    Immunopeptidomics:

    Article Title: Robust Bioconjugated Antigens Induce Immune Responses Preventing Malaria Infection and its Transmission.
    Article Snippet: The concentration of IFN-γ in the supernatants was easured using a Rat IFN gamma SimpleStep ELISAKit (Abcam, K), and the results were analyzed with a BioTek microplate eader. he stimulated and unstimulated BMDCs were collected and tained with 7-Aminoactinomycin D (7-AAD; Miltenyi Biotech, SA), following the manufacturer’s instructions, to identify he live cells. .. The cells were then stained with the following luorochrome-conjugated antibodies for 30 min at 4◦C: anti-rat D11c-APC-Vio770, CD80-PE, CD86-PE-Vio770, and MHC-IIerCP-Vio700 to assess the maturation of the dendritic cells. fter washing the cells twice with 1x PBS buffer, they were ixed with 1% paraformaldehyde (ThermoFisher Scientific, USA) nd analyzed using a flow cytometer within 24 h. All the ntibodies were purchased from Miltenyi Biotech, USA, and sed according to the manufacturer’s instructions. ..

    Flow Cytometry:

    Article Title: Robust Bioconjugated Antigens Induce Immune Responses Preventing Malaria Infection and its Transmission.
    Article Snippet: The concentration of IFN-γ in the supernatants was easured using a Rat IFN gamma SimpleStep ELISAKit (Abcam, K), and the results were analyzed with a BioTek microplate eader. he stimulated and unstimulated BMDCs were collected and tained with 7-Aminoactinomycin D (7-AAD; Miltenyi Biotech, SA), following the manufacturer’s instructions, to identify he live cells. .. The cells were then stained with the following luorochrome-conjugated antibodies for 30 min at 4◦C: anti-rat D11c-APC-Vio770, CD80-PE, CD86-PE-Vio770, and MHC-IIerCP-Vio700 to assess the maturation of the dendritic cells. fter washing the cells twice with 1x PBS buffer, they were ixed with 1% paraformaldehyde (ThermoFisher Scientific, USA) nd analyzed using a flow cytometer within 24 h. All the ntibodies were purchased from Miltenyi Biotech, USA, and sed according to the manufacturer’s instructions. ..



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    Peptide and cGAMP coacervates facilitate antigen cross-presentation. ( a ) Antigen cross-presentation of BMDCs treated with OVA-SIIN (10 μM), SIIN-4A (10 μM) and cGAMP (2 μM) in indicated formulations for 8 h and stained with PE-SIINFEKL antibody, and then recorded by confocal laser scanning microscope. ( b – e ) Antigen cross-presentation of BMDCs or BMDCs STING KO treatment as panel a, stained with APC-SIINFEKL antibody, and then detected by flow cytometry. ( b , d ) The red dashed line serves as a reference to clearly illustrate the degree of shift across different groups. ( f – h ) CD40, <t>CD80</t> and CD86 expression of BMDCs treated with OVA-SIIN (10 μM), SIIN-4A (10 μM) and cGAMP (2 μM) in indicated formulations for 24 h and detected by flow cytometry. Data are presented as mean ± SEM, n = 3 independent samples; ns, not significant, **** p < 0.0001 using one-way ANOVA with Tukey’s test.
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    Peptide and cGAMP coacervates facilitate antigen cross-presentation. ( a ) Antigen cross-presentation of BMDCs treated with OVA-SIIN (10 μM), SIIN-4A (10 μM) and cGAMP (2 μM) in indicated formulations for 8 h and stained with PE-SIINFEKL antibody, and then recorded by confocal laser scanning microscope. ( b – e ) Antigen cross-presentation of BMDCs or BMDCs STING KO treatment as panel a, stained with APC-SIINFEKL antibody, and then detected by flow cytometry. ( b , d ) The red dashed line serves as a reference to clearly illustrate the degree of shift across different groups. ( f – h ) CD40, CD80 and CD86 expression of BMDCs treated with OVA-SIIN (10 μM), SIIN-4A (10 μM) and cGAMP (2 μM) in indicated formulations for 24 h and detected by flow cytometry. Data are presented as mean ± SEM, n = 3 independent samples; ns, not significant, **** p < 0.0001 using one-way ANOVA with Tukey’s test.

    Journal: Vaccines

    Article Title: Peptide Coacervates Promote Cytosolic Delivery of STING Agonists for Cancer Immunotherapy

    doi: 10.3390/vaccines14040329

    Figure Lengend Snippet: Peptide and cGAMP coacervates facilitate antigen cross-presentation. ( a ) Antigen cross-presentation of BMDCs treated with OVA-SIIN (10 μM), SIIN-4A (10 μM) and cGAMP (2 μM) in indicated formulations for 8 h and stained with PE-SIINFEKL antibody, and then recorded by confocal laser scanning microscope. ( b – e ) Antigen cross-presentation of BMDCs or BMDCs STING KO treatment as panel a, stained with APC-SIINFEKL antibody, and then detected by flow cytometry. ( b , d ) The red dashed line serves as a reference to clearly illustrate the degree of shift across different groups. ( f – h ) CD40, CD80 and CD86 expression of BMDCs treated with OVA-SIIN (10 μM), SIIN-4A (10 μM) and cGAMP (2 μM) in indicated formulations for 24 h and detected by flow cytometry. Data are presented as mean ± SEM, n = 3 independent samples; ns, not significant, **** p < 0.0001 using one-way ANOVA with Tukey’s test.

    Article Snippet: ACK Lysis Buffer (E-CK-A105), mouse bone marrow-derived dendritic cells (BMDC) induction and identification kit (XJM003), including antibodies of Elab Fluor 488-CD11c, PE/Cyanine7-CD80, PE-CD86, APC-CD40, were purchased from Elabscience Biotechnology (Wuhan, China).

    Techniques: Staining, Laser-Scanning Microscopy, Flow Cytometry, Expressing